"Low intensity laser therapy: ¿possible strategy to restore fertility in premature ovarian failure induced by chemotherapy?"

DE ZÚÑIGA, I; SCOTTI, L; PASCUALI, N, HIGUERA, J ; FRAGOMENO, M; HORTON, M; KOPKOW, L; DI PIETRO M, IRUSTA, G; TESONE, M; ABRAMOVICH, D AND PARBORELL, F.

Congreso; 32nd Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE); 2016

ESHRE

"Low intensity laser therapy: ¿possible strategy to restore fertility in premature ovarian failure induced by chemotherapy?"de Zúñiga, I2; Scotti, L1; Pascuali, N1, Higuera, J1 ; Fragomeno, M1; Horton, M2; Kopkow, L2; Mariana Di Pietro1, Irusta, G1; Tesone, M1; Abramovich, D1 and Parborell, F11Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina. 2 PREGNA Medicina Reproductiva, Buenos Aires, Argentina, 3Fac. Cs Exactas y Naturales, UBA, Argentina.Premature Ovarian Failure (POF) is characterized by the disappearance of ovarian follicles in young women [1-3], which may be caused by chemotherapy [4;5]. POF treatments, which consist mainly of hormone therapies, are not completely effective. The present work proposes photobiomodulation as a strategy to protect the ovary and restore fertility in cancer patients undergoing chemotherapy by local application of low level laser therapy (LLLT) [6-9]. The objectives were: a) To evaluate the in vivo effect of LLLT in adult mice ovaries on follicular dynamics, b) To analyze the in vitro effect of LLLT in a culture of rat granulosa on cell proliferation and expression of VEGF, c) To evaluate the in vivo effect of LBI in the POF model induced by chemotherapy on ovarian folliculogenesis and expression of Anti-Müllerian Hormone (AMH). For objective a, F1 mice (BalbC x C57 / BL6) (8 weeks) were used and LLLT (2, 4 and 8 J/cm2) was applied. For objective b, a culture of rat granulosa cells (GC) (Sprague-Dawley, 21-23 days) was performed and LLLT (1-10 J/cm2) was applied. For the purpose c, POF model was induced with cyclophosphamide (CTX) in F1 mice and LLLT (8 J/cm2/ovary) was applied. The results showed that in adult mice the LLLT (4 and 8 J) increased ovarian reserve compared to control (p