Local VEGF inhibition prevents ovarian alterations associated with ovarian hyperstimulation syndrome

L. SCOTTI; D. ABRAMOVICH; N. PASCUALI; G. IRUSTA; G.MERESMAN; M. TESONE; F. PARBORELL

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2014

0960-0760

The relationship between human chorionic gonadotropin and ovarian hyperstimulation syndrome(OHSS) is partially mediated by vascular endothelial growth factor A (VEGF). The aim of this study was toinvestigate the effects of VEGF inhibition on the development of corpora lutea (CL) and cystic structures,steroidogenesis, apoptosis, cell proliferation, endothelial cell area, VEGF receptors (KDR and Flt-1),claudin-5 and occludin levels in ovaries from an OHSS rat model. The VEGF inhibitor used (VEGF receptor-1 (FLT-1)/Fc chimera, TRAP) decreased the concentrations of progesterone and estradiol as well as thepercentage of CL and cystic structures in OHSS rats, and increased apoptosis in CL. Endothelial cell area inCL and KDR expression and its phosphorylation were increased, whereas claudin-5 and occludin levelswere decreased in the OHSS compared to the control TRAP reversed these parameters. Ourfindingsindicate that VEGF inhibition prevents the early onset of OHSS and decreases its severity in rats.