INVESTIGADORES
PALMA Santiago Daniel
congresos y reuniones científicas
Título:
In Vitro Corneal Compatibility of Cross-Linked Hyaluronan-Itaconic Acid Films
Autor/es:
CALLES JAVIER; LOPEZ GARCIA; PALMA SANTIAGO; VALLES ENRIQUE; DIEBOLD YOLANDA
Lugar:
Viena
Reunión:
Simposio; The International Symposium on Ocular Pharmacology and Therapeutics; 2011
Institución organizadora:
ISOPT
Resumen:
Background: The aim of this work was to evaluate the biocompatibility between ocular surface cells and cross-linked Hyaluronic
Acid (HA)-Itaconic Acid (IT) films. Methods: HA-IT films were chemically modified using glutaraldehyde (GTA) and polyethylene
glycol diglycidyl ether (PEGDE) as crosslinking agents. Swelling behavior of cross-linked hydrogels and pH changes were studied in
culture medium. Structure and surface morphology were characterized by scanning electron microscopy (SEM). Suitability of
sterilization methods was studied including UV exposure and immersion in 70% ethanol. A human corneal epithelial cell (HCE) line
was used to determine the potential in vitro cell toxicity (XTT test) and cell proliferation rate alterations (Alamar Blue test) after
exposure to films for 24h. Results: Swelling assays indicated that cross-linked systems were capable of maintaining their integrity in
the aqueous medium during the length of experiments. All samples showed pores of different size by SEM. Culture medium pH
remained constant throughout the experiments. Sterilized PEGDE-exposed cells exhibited viability superior to 95% while sterilized
GTA cross-linked formulations lead into significant toxicity, as expected. Morphological details of PEGDE-exposed cells remained
intact. HCE cells proliferation rate was maintained after exposure to the different films for 24h with exception of HA control ones,
with which proliferation was slightly increased. GTA films-exposed cells showed a significant reduction in proliferation.
Conclusions/Discussion: Studied formulations had good physicochemical properties to obtain drug delivery films. The
biocompatibility assays showed no toxic effect and good HCE proliferation rates for PEGDE systems. These systems may be used
as topical drug delivery platforms. Support: CICYT MAT2010-20452-C03-01 (Spanish Ministry of Science and Tec