High-Frequency Somatic Embryogenesis with a Pampeana-Derived Genotype of Alfalfa (Medicago-Sativa L)

ROMAGNOLI, MARÍA VALERIA; ORTIZ, JPA; CERVIGNI, GD; HEISTERBORG, C; VALLEJOS, RH; JUAN PABLO AMELIO ORTIZ

EUPHYTICA

Kluwer Academic Publishers

Lugar: Amsterdam; Año: 1996 vol. 90 p. 89 - 93

Somatic embryos of genotype R11 of the alfalfa variety Pampeana were produced form embryogenic calli derived form leaf sections. They were induced by an auxin shock and its development was attenpted on six different media. The best conditions for somatic embryo production was inducing calli on MS medium plus 10 uM 2.4-D and 4.6 uM KIN and transferring them, after the auxin shock, to MS with 10.20 mM NH4+ and 30 mM proline. More than 500 somatic embryos per plate were produced. Embryos were grown to plants on MS or half strength MS media and all regenerated plants resembled the original R11 genotype. This technique could be useful in alfalfa Pampeana improvement using genetic modification.