INVESTIGADORES
ORTIZ Juan Pablo Amelio
artículos
Título:
Hygromycin Resistance as an Efficient Selectable Marker for Wheat Stable Transformation
Autor/es:
ORTIZ, JPA; REGGIARDO, MI; RAVIZZINI, RA; ALTABE, SG; CERVIGNI, GDL; SPITTELER, MA; MORATA, MM; ELIAS, FE; VALLEJOS, RH
Revista:
PLANT CELL REPORTS
Editorial:
Springer-Verlag
Referencias:
Lugar: Heidelberg; Año: 1996 vol. 15 p. 877 - 881
Resumen:
A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were by PCR amplification of transgene fragmtnts and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relatlvc to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.
