Endometrial inflammatory cytokines: the effects of milk production levels and hormonal context on local expression of them during early lactation of dairy cattle.

PERALTA MB; ANGELI, E; STASSI, A; CAINELLI, S; DURANTE, L; ORTEGA HH; REY F; SALINAS, F; VELAZQUEZ M

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Sociedad Argentina de Investigación Clínica

Dairy cattle undergo metabolic and hormonal changes that impactin the immunological system during peripartum. Previous reportssuggest that cows are immunosuppressed during postpartum andimmune components of the reproductive tract are altered. We analyzethe impact of milk production and the hormonal context on theexpression of endometrial cytokines. The animals were divided inhigh production group (HP: > 4800 L, n = 11) and low productiongroup (LP: < 4400 L, n = 13) considering the average of the dairyproduction up to 150 days in lactation. Blood samples and endometrialbiopsies were obtained at 45 and 60 days postpartum (dpp).The mRNA expression of interleukin IL1α, TNFα and IL6 was evaluatedby real time PCR. The protein expression was analyzed byimmunohistochemistry in the endometrial luminal epithelium (LE),glandular epithelium (GE) and stroma (S). Plasmatic concentrationsof cortisol, estrogen and progesterone were measured by ECLIA. Ageneral linear model was used to analyze the results. The resultsshowed that mRNA levels of IL1α were lower at 60 dpp than 45dpp, and LP group showed a decrease of IL1α mRNA expression inrelation to HP group (p<0,05). Also, the estradiol levels had significanteffect on the increase of IL1α mRNA expression. The mRNAlevels of TNFα showed no differences between analyzed groupsand the 75% of the samples showed no expression of IL6 mRNA.The protein expression of IL1α was significantly increased in LE underprogesterone effect (p<0,05). TNFα showed no differences inLE, GE and S between HP and LP groups (p>0,05). However, theprotein levels of IL6 in GE were significantly increased under theprogesterone effect. These results suggest that milk production andthe hormonal context affect the uterine cytokine expression duringevaluated postpartum period, especially under estradiol and progesteroneeffects.