ERYPTOSIS IS INDUCED BY HYPERTHERMIA IN HEREDITARY SPHEROCYTOSIS RED BLOOD CELLS

CRISP R; VOTA D; DONATO H; GARCÍA E; RAPETTI MC; MALTANERI R; VITTORI D; NESSE A

CLINICAL CHEMISTRY AND LABORATORY MEDICINE

WALTER DE GRUYTER & CO

Lugar: Berlin; Año: 2016 vol. 54 p. 165 - 168

1434-6621

ABSTRACTBackground:In patients with hereditary spherocytosis (HS) the development of hemolyticcrisis is recurrent during febrile infectious episodes. The aim of this workwas to evaluate whether the effect of temperature increment could affect HSerythrocyte survival.  Methods:Erythrocytes from HS patients and healthy donors were studied at 36.5 ºC and38.5 ºC, mimicking the physiologic body temperature and a febrile state,respectively. Flow cytometry was used to analyze signs of eryptosis, such ascell shrinkage (forward scatter) and phosphatidylserine translocation.Intracellular calcium content, reactive oxygen species and reduced glutathionelevels were determined by flow cytometry. Results:In in vitro assays, high temperature induced eryptosis in samples from HSpatients. No changes in the cellular oxidative status were observed due totemperature variation while increased intracellular calcium was detected in HSerythrocytes after incubation at 38.5 °C. Experiments at this temperaturecarried out in calcium-free media showed significantly decreased eryptosis. Conclusions:Our results show that high incubation temperature induces eryptosis of HSerythrocytes, which resulted more sensitive to temperature changes thancontrols. An increase in intracellular calcium seems to be an important factorresponsible for the eryptosis induced by high temperature and could explain theworsening of anemia during hyperthermia in HS patients.