Monoclonal Antibody Anti -Trypanosoma evansi Distinguishes Antigens Coming From Trypnosoma cruzi, , Babesia equi and Babesia caballi

MONZON,C.M

BIOCELL

Inca Editorial

Lugar: Mendoza; Año: 2004 vol. 28 p. 322 - 322

0327-9545

MONOCLONAL ANTIBODY ANTI-Trypanosoma evansi DISTINGUISHES ANTIGENS COMING FROM Trypanosoma cruzi, Babesia equi AND Babesia caballi   Monzón, C.M.   CONICET; Facultad de Ciencias de la Salud-U.Na.F., Formosa (Argentina), E-mail: cedivef@ciudad.com.ar       Trypanosoma evansi, T.cruzi, Babesia equi and B.caballi coexist in horses in the sub-tropical area of Argentina. The objective of this research was to study the cross antibody-antigen reaction between these protozoan parasites and evaluate the specificity of the monoclonal antibody 2-4F6 (Mab) against T.evansi. Using an indirect immunosorbent assay (ELISA) test, T.evansi antigens had positive reactions with T.cruzi and B.equi. This antigen only cross-reacted with T.evansi serum and surprisingly not with B.caballi. However B.caballi gave positive with B.equi and T.evansi sera. The Mab against T.evansi highly reacted with their homologous antigen but not with any of the heterologous antigens. In an ELISA inhibition test, double concentrations of T.evansi antigens from 4 to 512µg/ml of proteins, blocked between 84% and 92% the Mab activity, while the heterologous antigens employed at the same concentration did not produce inhibition. The antigen recognised by the Mab was not detected in the mentioned heterologous antigens using a double antibody sandwich ELISA technique for antigens detection.   The results in three different tests showed that the Mab 2-4F6 T.evansi did not react with T.cruzi, B.equi and B.caballi and could be useful to design a specific diagnostic test for T.evansi to be used in areas which in horses, these protozoan parasites coexist.