Multiplexed imaging of intracellular protein networks

GRECCO HE; IMTIAZ S; ZAMIR, E

CYTOMETRY PART A

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2016

1552-4922

Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed.