Effects on Ultrastructure, Composition and Specific Surface Area of the Gills of Odontesthes bonariensis Under Subchronic Glyphosate-Based Herbicide Exposure

RENATA J. MENÉNDEZ-HELMAN ; LEANDRO A. MIRANDA; ALFREDO SALIBIÁN ; MARÍA DOS SANTOS AFONSO

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

SPRINGER

Lugar: Berlin; Año: 2020

0007-4861

Gills represent one of the major sites of gas exchange of fsh, consequently they are in continuous close contact with the aquatic environment and its pollutants. In the present study the efects on gills of pejerrey fsh, Odontesthes bonariensis, under glyphosate-based herbicide subchronic exposure were analyzed. Adult animals were exposed to sublethal concentrations of a glyphosate-based commercial formulation (1 and 10 PMG mg L−1, PMG: glyphosate active ingredient) for 15 days, while control group was maintained in rearing water. Ultrastructural changes in gills were observed by scanning electron microscopy (SEM). The composition of the surface epithelium and specifc surface area were determined by energy dispersive spectroscopy (EDS) and N2 (g) adsorption?desorption isotherms, respectively. The herbicide exposure induced severealterations in gill ultrastructure, as shown in the SEM micrographs. Accordingly, an increase in surface area of the gills of exposed animals was determined. These results support that gills parameters of freshwater fsh are sensitive morphological biomarkers for glyphosate exposure.