INVESTIGADORES
MIRANDA Leandro Andres
artículos
Título:
Cloning of FSH-â, LH- â and glycoprotein hormone ásubunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRHs expression and plasma sex steroid levels in males fish.
Autor/es:
MIRANDA, LA; STRUSSMANN, CA; GUILGUR , LG; STROBL-MAZZULLA,, HP; SOMOZA,, GM
Revista:
JOURNAL OF FISH BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres ; Año: 2007 vol. 71 p. 1571 - 1571
ISSN:
0022-1112
Resumen:
Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone-b (FSH-b), luteinizing hormone-b (LH-b) and glycoprotein-a (GPH-a) subunits were cloned and characterized. Gene expression of these subunits was analysed by real-time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin-releasing hormones (GnRHs): Pacific salmon GnRH (GnRH-III), pejerrey GnRH (GnRH-I) and chicken GnRH-II (GnRH-II) and plasma sex steroid levels in adult males The nucleotide sequences of the FSH-b, LH-b and GPH-a subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH-b and GPH-a subunits, and intermediate levels of LH-b when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11-ketosterone (11-KT) values as well as low expression of sGnRH, cGnRH-II and pjGnRH. Running ripe males had the lowest expression of FSH-b and the highest expression of LH-b and GPH-a subunits, and of the three GnRH genes. At this stage, the highest values of T and 11-KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11-KT levels and cGnRH-II expression showed a tendency to decrease but the values were not statistically significant (P < 0_05) to running ripe stage. The present results would suggest that T and 11-KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH-b expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.