INVESTIGADORES
MIRANDA Leandro Andres
artículos
Título:
Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature-dependent sex Determination in pejerrey Odontesthes bonariensis.
Autor/es:
TAKAHIRO S; MIRANDA LA; OKUMA K; HATTORI RS; FERNANDINO JI; YOSHIZAKI G; SOMOZA GM; STRUSSMANN CA
Revista:
MOLECULAR REPRODUCTION AND DEVELOPMENT
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: Nueva York; Año: 2010 vol. 77 p. 521 - 532
ISSN:
1040-452X
Resumen:
In this study, we cloned and characterized the follicle stimulating hormone receptor
(Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes
bonariensis, a species with temperature-dependent sex determination (TSD), and
analyzed their expression in relation to Fshb and Lhb subunits during gonadogenesis
at temperatures producing only females (17C, FPT), both sexes (25C, MixPT), and
only males (29C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes
bonariensis, a species with temperature-dependent sex determination (TSD), and
analyzed their expression in relation to Fshb and Lhb subunits during gonadogenesis
at temperatures producing only females (17C, FPT), both sexes (25C, MixPT), and
only males (29C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey., a species with temperature-dependent sex determination (TSD), and
analyzed their expression in relation to Fshb and Lhb subunits during gonadogenesis
at temperatures producing only females (17C, FPT), both sexes (25C, MixPT), and
only males (29C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshb and Lhb subunits during gonadogenesis
at temperatures producing only females (17C, FPT), both sexes (25C, MixPT), and
only males (29C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.C, FPT), both sexes (25C, MixPT), and
only males (29C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of
694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Lhr cDNA had 2,936 bp for a
mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Lhr in fish
at the MPT, all genes showed significant increases and/or peaks of expression before
histological differentiation of the gonads regardless of temperature. Larvae at the FPT
had lower Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshb and Lhb but higher Lhr expression during theTSDperiod than those at
the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshr. At the MixPT, Fshb, Lhb,
and Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Lhr mRNA increased in approximately half of the fish during TSD and sex
differentiation and the sex ratio was 55.2% male. Based on the above results, it is
suggested that animals with high Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshb and Lhb and low Lhr values represent putative
males. These evidences, together with other studies, suggest that temperature may
signal through the pituitary (differential expression of Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Fshb and Lhb) down to the
gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.Lhr), probably affecting the regulation of steroidogenesis
during the TSD process of pejerrey.