INVESTIGADORES
MIGLIORANZA Karina Silvia Beatriz
congresos y reuniones científicas
Título:
Can the oxidative stress responses in pine needles be used as reliable biomarkers to assess exposure to POPs?
Autor/es:
KARINA S. B. MIGLIORANZA; GONZALEZ MARIANA; ONDARZA PAOLA MARIANA; MENONE MIRTA; MITTON, FRANCESCA; LUKASZEWICKS GERMAN; SHIMABUKURO VALERIA; FILLMANN GILBERTO
Lugar:
Indianapolis
Reunión:
Congreso; 246th ACS National Meeting & Exposition en Indianapolis; 2013
Resumen:
Anthropogenic contaminants are atmospherically
transported and passive air sampling, is increasingly being recognized as an effective approach for measuring the atmpsphere relative
concentrations of Persistent Organic Pollutants (POPs) such as organochlorine
pesticides (OCPs),
polychlorinated biphenyls (PCBs) and polybrominated diphenyls ethers (PBDEs). Pine needles accumulate POPs in their tissues and integrate contaminants over a long time. POPs
are able to generate Reactive
Oxygen Species (ROS), but the
organisms have evolved systems of antioxidant defense. This project seeks to study the combined effects of environmental pollutants
on oxidative stress biomarker systems to determine whether some of those
changes could be used as reliable biomarkers to assess the end results of
exposure to POPs. Pine needles
were used to evaluate longitudinal and latitudinal POPs levels in the atmosphere
of Patagonia, Argentina. Levels of lipid peroxidation, GST activity and total
antioxidant capacity (ACAP) were also evaluated. HCHs, endosulfans, DDTs, PCBs
and PBDEs congeners were determined The Rio Negro watershed, characterized by
an intensive fruit production, showed the highest POPs levels with a
predominance of endosulfans (51-90%) as result of its unrestricted use,
followed by DDE and low proportion of PCBs and PBDE; concomitantly high ACAP
and GST activity were quantified. A decrease gradient in POPs and biomarkers
levels from west to east was observed, showing a direct relation between both
parameters. An increasing latitudinal gradient (20-440 ng/g lipid weight) to
the southeast station (Rio Gallegos) with a predominance of DDTs (30%), PCBs
(35%) and PBDEs was observed but the non-direct relationship with biomarkers would
be influenced by other factors, both biochemicals or environmental conditions in
biomarker responses to POPs. Thus, the biomarkers selected would be a useful
tool in the atmospheric pollution assessment by POPs but more studies
considering pine needle lipid classes and a range of temperature among stations
should be performed.