Uptake, metabolism and sublethal effects of BDE-47 in two estuarine invertebrates with different trophic position.

DIAZ JARAMILLO MAURICIO; MIGLIORANZA KARINA SILVIA BEATRIZ; GONZALEZ MARIANA; BARON E; MONSERRAT JOSE M; ELJARRAT EHEL; BARCELO DAMIA

ENVIRONMENTAL POLLUTION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2016 vol. 213 p. 608 - 617

0269-7491

Two microcosm types -sediment-biota and biota-biota- were constructed to simulate different pathwaysof BDE-47 uptake, metabolism and oxidative stress effects in two key estuarine invertebrates (polychaeteLaeonereis acuta and crab Cyrtograpsus angulatus). In the sediment-biota experiment, both species wereexposed to spiked sediments; an environmentally reported and a high concentration of BDE-47 for 2weeks. In the biota-biota experiment, crabs were fed with polychaetes pre-exposed to BDE-47 in thesediment-biota experiment. The sediment-biota experiment first revealed that polychaetes significantlyaccumulated BDE-47 (biota-sediment accumulation factor >2; p < 0.05) to a much greater extent thanthe crab organs (muscle, hepatopancreas, gills) at both sediment concentrations. For oxidative stressresponses, polychaete and crab tissues exposed to spiked sediment showed a significant increase(p < 0.05) of only glutathione S-transferase (GST) activity with respect to controls in both BDE-47concentrations. No lipid peroxidation (TBARS) or total antioxidant capacity (ACAP) changes wereevident in the species or organs exposed to either BDE-47 sediment concentration. The biota-biotaexperiment showed that feeding crabs with pre-exposed polychaetes caused BDE-47 accumulation inorgans as well as significant amounts of BDE-47 eliminated through feces (p < 0.05). Unlike thesediment-biota exposure, crabs fed with pre-exposed BDE-47 polychaetes showed the most conspicuousoxidative stress responses. Significant changes in GST and ACAP in both hepatopancreas and gills, inaddition to enhanced TBARS levels in the hepatopancreas with respect to controls (p < 0.05), revealedthat BDE-47 assimilated by invertebrates represents a potential source of toxicity to their predators. Nomethoxylated metabolites (MeO-PBDEs) were detected during BDE-47 metabolism in the invertebratesin either of the two different exposure types. In contrast, hydroxylated metabolites (OH-PBDEs) weredetected in polychaetes and crab organs/feces in both experiments. Our results demonstrate that PBDEhydroxylation is one of the main biotransformation routes of BDE-47 in estuarine animals, which couldbe associated with the oxidative stress responses found.