INVESTIGADORES
MAYORGA Luis Segundo
congresos y reuniones científicas
Título:
Calcium signaling in human spermatozoa.
Autor/es:
DE BLAS, G.; BRANHAM, M.T; TREVINO, CL; SOLIS A; TOMES, CN; MAYORGA, LS; DARSZON, A.
Reunión:
Congreso; 48º SAIB; 2012
Institución organizadora:
SAIB
Resumen:
Calcium and cAMP signaling plays a pivotal role in sperm
physiology, being intimately involved in the regulation of
capacitation, hyperactivation and acrosome reaction. In a previous
report we have proposed that the cAMP analogue 8-(pchlorophenylthio)-2'-O-methyladenosine-3', 5'-cyclic
monophosphate (8-pCPT-2'-O-Me-cAMP) elicits intracellular
calcium release in human sperm. Intracellular calcium stores play a
central role in the regulation of cellular [Ca2+]i and in the
generation of complex [Ca2+] signals such as oscillations and
waves. The aim of this study was to investigate the calcium stores
and calcium channels involved in the 8-pCPT-2'-O-Me-cAMP
pathway in human spermatozoa. We used real time dynamic assays -
with high speed and spatial resolution- and fluorescent calcium
sensors. To asses this, capacitated human sperm were loaded with
Fluo3-AM, then incubated in a calcium-free medium and treated
with different agonist of the AR (8-pCPT-2'-O-Me-cAMP,
progesterone and recombinant human ZP3). We found that all AR
inducers triggered spatial distribution of Ca2+and using a kinetic
assay we studied the dynamics and direction of the calcium wave.
Our results indicate that 8-pCPT-2'-O-Me-cAMP, progesterone and
ZP3 induce a calcium wave that starts in the posterior neck region
and propagates to the acrosome region.