INVESTIGADORES
MATTION Nora Marta
artículos
Título:
Alterations in the sequence of the gene 4 from a human rotavirus after multiple passages in HepG2 liver cells
Autor/es:
KITAMOTO, NORITOSHI; MATTION, NORA; ESTES, MARY
Revista:
ARCHIVES OF VIROLOGY
Editorial:
Springer Wien
Referencias:
Lugar: new York; Año: 1993 vol. 130 p. 179 - 185
ISSN:
0304-8608
Resumen:
The human Wa strain of rotaviruses, initially unable to grow in liver cells, was adapted by multiple passages to grow in HepG2 cells. The genome segment 4 of both the parental and passaged strains was cloned and sequenced. Five amino acid differences (residues 38, 120, 421, 525, and 618) were found in the HepG2-passaged variant compared to the parental Wa strain. Our results support the hypothesis that viral variants that have improved capabilities for infecting liver cells can be generated during infection
