INVESTIGADORES
MALANGA Gabriela Fabiana
artículos
Título:
Oxidative Effects of Glyphosate on the Lipophobic Intracellular Environment in the Microalgae Chlorella Vulgaris
Autor/es:
JM OSTERA; S PUNTARULO; G MALANGA
Revista:
BIOCELL - Tech Science Press
Editorial:
tech science press
Referencias:
Año: 2021
ISSN:
1667-5746
Resumen:
Abstract: The studied hypothesis is that the herbicide glyphosate (GLY) can affect the oxidative balance in the hydrophobic intracellular medium in non-target Chlorella vulgaris cells. Analytical GLY and RoundUp (RUP) supplementation, affected the growth profile. A significant 42% decrease in the cellular biomass in stationary (St) phase was observed in cultures supplemented with either 5 μM of GLY or RUP, as compared to control cultures. The treatment with 0.3 μM of GLY generated non-significant effects on the oxidation rate of 2?, 7? dichlorofluorescein diacetate (DCFH-DA), neither in exponential (Exp) nor in St phase of development, as compared to control cultures.However, the treatment with either 5 μM GLY or 0.3 and 5 μM RUP lead to a significant decrease in the DCFH-DA oxidation rate, as compared to control cultures. The lipid radical (LR●) generation rate, detected by Paramagnetic Resonance Spectroscopy (EPR), was significantly increased in the presence of RUP, in Lag and Exp phase of growth. The non-enzymatic antioxidants, α-Tocopherol (α-T) and β-Carotene (β-C), are aimed to protect membranes against the damage produced by the radical reactions. The content of β-C was not significantly affected, as compared to control cultures, by any of the treatments, in both growth phases of cellular development. The content of α-T wassignificantly decreased by the supplementation with either 0.3 or 5 μM of RUP or 5 μM GLY. The LR●/α-T ratio, used as indicator of the oxidative balance in the hydrophobic cellular media, was significantly different between samples obtained from control and RUP-exposed microalgae in both, Exp and St phase of development, with either 0.3 or 5 μM RUP. The data presented here showed evidence that suggested that oxidative balance in the hydrophobic environment was affected by either GLY or RUP.
