INVESTIGADORES
LOZANO Mario Enrique
congresos y reuniones científicas
Título:
Development of a production system for Z VLPs in insect cells
Autor/es:
BORIO, C.S.; BILEN, M.F.; LOZANO, M.E.
Lugar:
Potrero de los Funes, San Luis
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
The
assembly of proteins as virus-like particles (VLPs) using protein expression
technology in yeast, insect and mammalian cells is a highly effective type of
subunit vaccine, already tested and approved. The Arenavirus´s Z protein is
involved in the assembly and budding of virions from the cell membrane. It also
has been reported that the only expression of Z is sufficient for the release
of Z-containing particles of Lassa Fever Virus. The generation of this VLPs
from Z expression could have different biotechnological applications, such as
drug and antigen delivery. Because of this is necessary the development of a
safe, efficient and easy to scale up VLPs production system. Insect cells are a
suitable candidate, since they express large amounts of correctly folded
recombinant proteins and have been used for previously approved vaccines, as
Cervarix. In order to determine if Junin virus Z protein maintains its budding capacity
on insect cells, the recombinant Z-EGFP protein was expressed on Sf9 insect
cells using the Bac-to-Bac system. We analyzed the culture supernatant of
infected cells expressing ZEGFP and we detected its presence in particles
obtained by ultracentrifugation. Also these particles were preserved from degradation
of proteinase K by a lipid membrane.
These
results suggest the presence of Z-EGFP in particles obtained from the culture
supernatant of infected cells.
