Biosynthesis of antiviral compounds using immobilized thermophilic bacteria

DE BENEDETTI, E.; RIVERO, C.; LOZANO, M. E.; TRELLES, J.A.

Ciudad de Mendoza

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2012

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Biocatalysis is widely used in replacement of many chemical reactions which requires several steps of protection and deprotection and the use of toxic solvents, obtaining racemic mixtures as final products. Particularly, thermophilic microorganisms are very useful in industrial bioprocesses due to the high stability of their enzymes. The application of immobilization techniques improve biocatalyst reusability, simplify product recovery and allow bioprocess scale-up.In this work, we describe the use of thermophilic bacteria as biocatalysts for the biosynthesis of nucleoside analogues with antiviral or antitumoral activity. Different parameters such amount of microorganisms, substrate ratio, temperature and culture time were evaluated. 2,6-diaminopurine and 6-chloropurine ribosideswere obtained with conversion rates of 83% and 68%, respectively at short reaction times. The conversion values for 2,6-diaminopurine and 6-chloropurine-2´-deoxyribosides were 90%.Besides, this biocatalyst was able to biosynthesize 6-bromopurine and 6-iodopurine nucleosides with high yields.Finally, the biocatalyst immobilized in agarose matrix was active for more than 200 h obtained 23.4 g of nucleoside analogues.