INVESTIGADORES
LOZANO Mario Enrique
artículos
Título:
Biosynthesis of anti-HCV compounds using thermophilic microorganisms
Autor/es:
RIVERO, C.; DE BENEDETTI, E.; SAMBETH, J.; LOZANO, M.E.; TRELLES, J.A.
Revista:
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 22 p. 6059 - 6062
ISSN:
0960-894X
Resumen:
This work describes the application of thermophilic
microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of
6-chloropurine-2'-deoxyriboside and 6-chloropurine riboside was achieved by
Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%,
respectively. Furthermore, the selected microorganism was satisfactorily
stabilized by immobilization in an agarose matrix. This biocatalyst can be reused
at least 70 times without significant loss of activity, obtaining 379mg/L of
6-chloropurine-2'-deoxyriboside. The obtained compounds can be used as
antiviral agents.
