Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria

BRITOS, C.; CAPPA, V.; RIVERO, C.; SAMBETH, J.; LOZANO, M.E.; TRELLES, J.A.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 79 p. 49 - 53

1381-1177

An efficient and green bioprocess is herein reported to obtain halogenatednucleosides by transglycosylation using immobilized lactic acid bacteria (LAB).Lactobacillus animalis ATCC 35046 showed a yield of 95 % at 0.5 h to synthesize 5-fluorouracil-2?-deoxyriboside (floxuridine). Calcium alginate was the best matrix forwhole-cell immobilization by entrapment. Its productivity was 87 mg/Lh in a continuousbioprocess. When adsorption techniques were evaluated, DEAE-Sepharose was thesupport which showed higher microbial load, its productivity being 53 mg/Lh.Additionally, this microorganism was able to produce 5-bromouracil-2´-deoxyriboside,6-chloropurine-2´-deoxyriboside and 6-bromopurine-2´-deoxyriboside.