Green biosynthesis of floxuridine by immobilized microorganisms

RIVERO, C.; BRITOS, C.; LOZANO, M. E.; SINISTERRA, J.; TRELLES, J.A.

FEMS MICROBIOLOGY LETTERS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 331 p. 31 - 36

0378-1097

This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2′-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L−1 min−1 (60%) of 5-chloro-2′-deoxyuridine in 3 h. These halogenated pyrimidine 2′-deoxynucleosides are used as antitumoral agents.