INVESTIGADORES
LOZANO Mario Enrique
artículos
Título:
Characterization of arenavirus using a family-specific primer set for RT-PCR amplification and RFLP analysis. Its potential use for detection of uncharacterized arenaviruses
Autor/es:
LOZANO, M.E.; POSIK, D.M.; ALBARIÑO, C.G.; SCHUJMAN, G.; GHIRINGHELLI, P.D.; CALDERÓN, G.; SABATTINI; M.S.; ROMANOWSKI, V.
Revista:
VIRUS RESEARCH
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Maryland, MO, EEUU; Año: 1997 vol. 49 p. 79 - 89
ISSN:
0168-1702
Resumen:
Arenaviruses are enveloped viruses with a genome composed of two ssRNA
species, designated L and S. The arenaviruses were divided in two major
groups (Old World and New World), based on serological properties and
genetic data, as well as geographic distribution. A sequence alignment
analysis of all reported arenavirus S RNAs yielded 17 conserved regions
in addition to a reported conserved region at the end of both RNAs. The
consensus sequences of these regions were used to design generalized
primers suitable for RT-PCR amplification of a set of overlapping
nucleotide sequence fragments comprising the complete S RNA of any
arenavirus. A restriction analysis (RFLP) was designed to rapidly typify
the amplified fragments. This RT-PCR-RFLP approach was tested with Old
World (LCM) and New World (Junin and Tacaribe) arenaviruses.
Furthermore, using this procedure the whole S RNA of a novel arenavirus
isolate obtained from a rodent trapped in central Argentina, was
amplified and characterized. Partial nucleotide sequence data were used
for phylogenetic analyses that showed the relationships between this
arenavirus and the rest of the members of the family. This relatively
simple methodology will be useful both in basic studies and
epidemiological survey programs.