°Sucrase and maltase activities in hepatopancreas of Neohelice granulata: Post-ingesta response¡±

ASARO, A.; LÓPEZ MAÑANES, A,A.; DEL VALLE, J.C

Buenos Aires

Jornada; X Annual Meeting of Argentine Biology Society.; 2008

Hepatopancreas has a central role in crustacean digestive physiology. Studies on key digestives enzymes in euryhaline crabs are lacking. The aim of this work was to study biochemical characteristics and post-ingesta response of sucrase (Suc) and maltase (Mal) activities in hepatopancreas of N. granulata. Adult males were acclimated 14 days in 35¢¶ salinity. The supernatant (10000xg 15min) from an hepatopancreas homogenate (0,1M Tris-HCl, pH 7,4) (4 ml buffer x g of tissue-1) was used. Suc and Mal were assayed by hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as 14 days in 35¢¶ salinity. The supernatant (10000xg 15min) from an hepatopancreas homogenate (0,1M Tris-HCl, pH 7,4) (4 ml buffer x g of tissue-1) was used. Suc and Mal were assayed by hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as 14 days in 35¢¶ salinity. The supernatant (10000xg 15min) from an hepatopancreas homogenate (0,1M Tris-HCl, pH 7,4) (4 ml buffer x g of tissue-1) was used. Suc and Mal were assayed by hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as N. granulata. Adult males were acclimated 14 days in 35¢¶ salinity. The supernatant (10000xg 15min) from an hepatopancreas homogenate (0,1M Tris-HCl, pH 7,4) (4 ml buffer x g of tissue-1) was used. Suc and Mal were assayed by hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as -1) was used. Suc and Mal were assayed by hydrolysis of the corresponding substrate in 0.1 M maleate/OHNa 30¨¬C (pH curve: 3.5-8.3, S=28mM; substrate curve: S=0.56-42 mM, pH=6.4; ingesta: S=28 mM, pH=6.4). To study post-ingesta response, crabs were starved for 5 days (t0). Suc and Mal activities were assayed 2 and 4 h post-ingesta. The activity was expressed as ¥ìg glucosa x mg prot-1 x min-1. Suc and Mal activities were similar at pH range 3.5-6.8, being 27-32% lower at pH 8 and exhibited Michaelis-Menten kinetics (Km(mM)=2.5 and 5.1). Suc and Mal did not vary after ingesta (Suc:t0=77.3¡¾22.7, t2=67.4¡¾17.2, t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. at pH range 3.5-6.8, being 27-32% lower at pH 8 and exhibited Michaelis-Menten kinetics (Km(mM)=2.5 and 5.1). Suc and Mal did not vary after ingesta (Suc:t0=77.3¡¾22.7, t2=67.4¡¾17.2, t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. at pH range 3.5-6.8, being 27-32% lower at pH 8 and exhibited Michaelis-Menten kinetics (Km(mM)=2.5 and 5.1). Suc and Mal did not vary after ingesta (Suc:t0=77.3¡¾22.7, t2=67.4¡¾17.2, t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. g glucosa x mg prot-1 x min-1. Suc and Mal activities were similar at pH range 3.5-6.8, being 27-32% lower at pH 8 and exhibited Michaelis-Menten kinetics (Km(mM)=2.5 and 5.1). Suc and Mal did not vary after ingesta (Suc:t0=77.3¡¾22.7, t2=67.4¡¾17.2, t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. ¡¾22.7, t2=67.4¡¾17.2, t4=55.9¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. ¡¾9) (Mal: t0=539.3¡¾116.1, t2=406.0¡¾102.5, t4=319¡¾54.9) (ANOVA, p>0.05). At t0 Mal activity was Suc activity-dependent (p<0.05) whereas at t2 and t4 post-ingesta it was Suc-independent (p>0.05). The results show that N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity. N. granulata hepatopancreas exhibits Suc and Mal activities and suggest a cualitative modulation post-ingesta of maltase activity.