Glycogen storage sites in Chasmagnathus granulatus upon hyperegulation: differential postingesta response

ARTILLO GUIDA R.; PINONI,S; ASARO, A.; LÓPEZ MAÑANES A.

Córdoba, Argentina

Congreso; First Meeting of Argentine Biology Societies; 2007

Sociedades Argentinas de Biología

Glycogen (G) storage sites in crustacean decapods vary depending on species, physiological status and stress condition. The aim of this work was to identify G storage tissues of C. granulatus at different times post ingesta upon hyperegulation conditions. Adult male crabs acclimated for 14 days at 10 ‰ salinity were starved for 5 days. After starvation (t0) crabs were fed. At t0 and up to 92 h post ingesta G (mg glucose x g tissue-1) was quantified in homogenates from chela muscle (M), hepatopancreas (HP) and anterior gills (AG) by hydrolysis with á-amyloglucosidase (Sigma Chemicals) (0.2ml/ ml) for 2,5 h at 55°C. Released glucose from G was determined with a commercial kit (Wiener-Lab AA). Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). At t0 G content was: HP=2.9±1.1, M=32.3±5.0, AG=20.6±1.3. At 23 and 92 h G content increased in GA (34.8±3.5 and 3850±3.3, respectively). At 92 hs G content increased in HP(11.3±2.6). No changes occurred in either M or HP. Our results show that in C. granulatus anterior gills constitute G storage sites after ingesta.