Effect of diets with diferent lipid conTent in the crab Neohelice granulata from different habitats

MENDEZ, E.; LÓPEZ MAÑANES, A,A.

Encuentro; XV Annual Meeting Argentine Biology Society; 2013

The animals undergoing dietary changes among habitats are expected to exhibit metabolic adjustments. However intraespecfics studies in estuarine euryhaline crabs are lacking. Objective: To study the effect of diets with different lipid proportions on triglycerides (TG) and protein (Prot) content in hepatopancreas (HP) of individuals of N. granulata from mudflat (MF) and saltmarsh (SM) of the Mar Chiquita lagoon (Pcia. of Bs As). Methods: Adult male crabs were fed 3 times a week (diets with 3,5 (D1) or 5 % (D2) of lipids) and starved 48 h before determinations. Before (t0) and after 30 (t30) days, TG (mg tissue xg-1) and Prot (mgtissuexg-1) were determined in HP homogenate (0.1M Tris-HCl, pH 7.4, 4mlxgtissue-1) by enzyme kit TAG Wiener-Lab.AA and Bradford method, respectively. Results: Individuals from MF: D1 (TG: 460.33 ± 285.54; Prot: 23.61 ± 2.58) and D2 (TG: 242.88 ± 35.01; Prot: 26.08 ± 3.38) did not affect TG or Prot content. In individuals from SM: Prot was higher (660%) than t0 (18.27 ± 1.5) in subjects fed with D1. TG did not change. There was no change with D2. Conclusions: The differential responses in Prot content in HP regarding diets with different lipid proportion suggests the existence of differential metabolic adjustments relative to the habitat and compensatory adjustments and interrelation within different metabolic pathways