Integrative study at the biochemical level in a intertidal crab from Mar Chiquita coastal lagoon (Buenos Aires, Argentina) upon acute exposure to high zinc

PANELO.L.; PANZERI,A.; DEL VALLE, J.C; LÓPEZ MAÑANES, A,A.

Pan-American Journal of Aquatic Sciences

Pablo Muniz

Año: 2015 vol. 10 p. 55 - 67

1809-9009

Abstract In Mar Chiquita coastal lagoon (Argentina) acute high increases in Zinc (Zn) occur reaching 1.22 mg x L-1, but integrative studies on possible effects at the biochemical-physiological level on conspicuous invertebrate species inhabiting this area are lacking. We determined body and vital organs mass, hyper/ hypo-regulatory capacity; Na+-K+ATPase activity alkaline phosphatases (AP) activities in chela muscle, maltase and sucrase activities in the hepatopancreas and glycemia of adult males of the euryhaline crab Neohelice granulata upon exposure during 96 h to 1.22 mg x L-1 Zn in hyper- and hypo-osmotic media. Body, anterior and posterior gills, hepatopancreas and chela muscle mass and hyper and hypo-regulatory capacities were not affected suggesting maintenance of body and physiological condition. The fact that upon Zn exposure in hypo-osmotic media Na+-K+ATPase activity decreased in posterior gills while increased in anterior gills along with increased glycemia suggests the occurrence of biochemical compensatory adjustments. Since no changes occurred in maltase and sucrase activities in the hepatopancreas or AP in chela muscle in hypo-osmotic media, other key metabolic parameters appeared not to be comprised. The fact that in hyper-osmotic media Zn exposure no effects were found suggests the occurrence of specific effects of Zn along upon a hypo-osmotic challenge