EFFECT OF METHYL FARNESOATE, ALONE AND IN COMBINATION WITH OTHER HORMONES, ON OVARIAN GROWTH OF THE RED SWAMP CRAYFISH, PROCAMBARUS CLARKII, DURING VITELLOGENESIS

RODRIGUEZ, E.M, LOPEZ GRECO, L.S, MEDESANI, D.A, LAUFER, H. Y M. FINGERMAN

GENERAL AND COMPARATIVE ENDOCRINOLOGY

ELSEVIER SCIENCE

Año: 2002 vol. 125 p. 34 - 40

0016-6480

E.M. RODRIGUEZ, L.S. LOPEZ GRECO, D.A. MEDESANI, H. LAUFER y M. FINGERMAN. 2002. General and Comparative Endocrinology 125: 34-40 The effect of methyl farnesoate (MF) on the ovaries of female red swamp crayfish, Procambarus clarkii, undergoing vitellogenesis was determined both in vivo and in vitro. The in vivo assay showed a positive effect of MF on oocyte growth when injected alone and in combination with 17©¬-estradiol, but not in combination with JHIII or 17¥á-hydroxyprogesterone. A higher level of incorporation of labelled leucine was induced by MF on isolated pieces of ovary. The same effect was seen when ovary and mandibular organ (MO) were coincubated. This results suggest that MF stimulated the synthesis of vitellin in the ovary of crayfish. In vitro, 17¥á-hydroxyprogesterone completely suppressed the stimulatory action of the MO on the ovary, suggesting a competitive inhibition between 17¥á-hydroxyprogesterone and MF on the ovary and/or a negative feedback by that steroid on the MO