INVESTIGADORES
LODEIRO Anibal
artículos
Título:
Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
Autor/es:
ALTHABEGOITI, MJ; COVELLI, JM; PÉREZ-GIMÉNEZ J; QUELAS JI; MONGIARDINI EJ; LÓPEZ MF; LÓPEZ-GARCÍA SL; LODEIRO AR
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2011 vol. 319 p. 133 - 139
ISSN:
0378-1097
Resumen:
Bradyrhizobium japonicum has two types of flagella. One has thin filaments
consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has
thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4
(FliC1-4). To investigate the roles of each flagellum in competition for nodulation,
we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds
of two derivatives from the reference strain USDA 110: the streptomycin-resistant
derivative LP 3004 and its more motile derivative LP 3008. All mutations
diminished swimming motility. When each mutant was co-inoculated with the
parental strain on soybean plants cultivated in vermiculite either at field capacity
or flooded, their competitiveness differed according to the flagellin altered. DfliCIIIhas two types of flagella. One has thin filaments
consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has
thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4
(FliC1-4). To investigate the roles of each flagellum in competition for nodulation,
we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds
of two derivatives from the reference strain USDA 110: the streptomycin-resistant
derivative LP 3004 and its more motile derivative LP 3008. All mutations
diminished swimming motility. When each mutant was co-inoculated with the
parental strain on soybean plants cultivated in vermiculite either at field capacity
or flooded, their competitiveness differed according to the flagellin altered. DfliCIIIfliCI-II and/or fliC1-4 in the genomic backgrounds
of two derivatives from the reference strain USDA 110: the streptomycin-resistant
derivative LP 3004 and its more motile derivative LP 3008. All mutations
diminished swimming motility. When each mutant was co-inoculated with the
parental strain on soybean plants cultivated in vermiculite either at field capacity
or flooded, their competitiveness differed according to the flagellin altered. DfliCIIIDfliCIII
mutants were more competitive, occupying 6480% of the nodules, while
DfliC1-4 mutants occupied 4549% of the nodules. Occupation by the nonmotile
double mutant decreased from 55% to 11% as the water content of the vermiculite
increased from 85% to 95% field capacity to flooding. These results indicate that
the influence of motility on competitiveness depended on the water status of the
rooting substrate.fliC1-4 mutants occupied 4549% of the nodules. Occupation by the nonmotile
double mutant decreased from 55% to 11% as the water content of the vermiculite
increased from 85% to 95% field capacity to flooding. These results indicate that
the influence of motility on competitiveness depended on the water status of the
rooting substrate.