Renal cdc42 and CIP4 expression in an ischemia-reperfusion injury model.

FUSSI MF; TONUCCI F; VIGNOLA J; MARQUEZ SB; GIRARDINI J; MONASTEROLO L; TRUMPER L; LAROCCA MC; MOLINAS S

Buenos Aires

Congreso; Reunion cientifica anual conjunta de Sociedades de Biociencias; 2017

Acute kidney injury (AKI) is still a significant medical problem. Renalischemia-reperfusion (IR) injury is one of the main causes of AKI.Cdc42 is a master signaling molecule, involved in the maintenanceof epithelial integrity in renal tubules. Cdc42 signals the initial eventsof the epithelial mesenchymal transition (EMT), which is essentialin the response of the injured kidney. Our aim was to contribute tothe molecular characterization of the signaling pathways involvedin the development of AKI by characterizing specific features of thecdc42 pathway in the IR model. Male Wistar rats (n=6 per group)were subjected to 40 min of unilateral renal ischemia followed by1 (IR1) or 7 (IR7) days of reperfusion. Controls underwent shamoperation. AKI was corroborated by histological and functional studies.Glomerular filtration rate was reduced in IR1 (-54%, p