CORRELATION BETWEEN P-REX1 WITH CLINICAL AND HISTOPATHOLOGICAL FEATURES IN BREAST CANCER PATIENTS

LARA, ANGELA; GONZALEZ NAZARENO; CICINELLI KARINA; JORDANOVSKI NOEMI; COSTA FLORENCIA; GON CARINA; CARRASCO MARY; MALTAGLIATTI DANIELA; SANCHOTENA VERÓNICA; LEGUINA LAURA; VORNETTI SILVIA; FLAKS DIEGO; ACOSTA GABRIELA; CUNEO NICASIO; ABBA MARTÍN; EDITH C. KORDON; LORENZANO PABLO ; ARIAS CLAUDIA; WERTHEIMER EVA; DE LAURENTIIS

CABA

Congreso; Reunión Anual SAIC 2021; 2021

Breast cancer is a multifactorial disease and current therapies oftenfail. At present, treatment decisions should be based on the underlyingmolecular pathways and biological mechanisms. P-Rex1 is aRac-GEF essential for Rac1 activation that plays an important rolein regulating cell migration. It has been reported that P-Rex1 is overexpressedin luminal breast tumors and, when silenced, the migrationof cancer cells is inhibited. We evaluated the levels of P-Rex1and its association with clinical- histopathological parameters inArgentine patients with breast cancer at the Marie Curie OncologyHospital. We analyzed the expression of P-Rex1 in biopsy materialand in tumors and their corresponding healthy adjacent tissues frompatients not subjected to neoadjuvant therapy. P-REX1 mRNA expressionwas analyzed by quantitative PCR (Q- PCR), and proteinby Western blot (WB) or immunohistochemistry (IHC). These datawere validated by an in silico analysis made from public access databases(Metabric, GEO repository of NCBI and UCSC Xena).P-REX1 mRNA levels were significantly overexpressed (p