ISLA Maria Ines
congresos y reuniones científicas
Antioxidant activity of a chalcone isolated from Amaicha del Valle propolis, Tucumán
NIEVA MORENO M.I., ZAMPINI I.C., ORDOÑEZ R.M., VATTUONE M.A., ISLA M.I.
Congreso; XXI Jornadas de la Asociación de Biología de Tucumán; 2004
Asociación de Biología de Tucumán
We have previously demonstrated that North Argentine propolis exhibit protective action against oxidative modification of lipid and like wise free radical and reactive oxygen species scavenging activities do. These results justify the use of propolis as a possible source of a natural antioxidant. Recently, we isolated from Amaicha del Valle propolis a bioactive compound that was identified as 2,4 dihydroxychalcone. The purpose of this work was to compare the antioxidant activities between propolis extract and the bioactive compound using the b-Carotene-linoleic acid assay. The Amaicha del Valle propolis exhibit antioxidant activity, by protective action on b-caroteno termic oxidation. The flavonoids concentration in propolis extract required for exerting the 50 % of protective activity was 0.4 µg/ml. The TLC chromatograms revealed with ß-carotene linolate system, also confirm the antioxidant activity of the bioactive compound. Its antioxidant activity is similar to quercetin, a powerfull natural antioxidant (0.3 µg/ml). Our results suggest a potential use of Amaicha del Valle propolis as phytopharmaceutic products and for food conservation.