INVESTIGADORES
ISLA Maria Ines
artículos
Título:
Antifungal, anti-inflammatory and antioxidant activity of bi-herbal phytotherapic using medicinal plants from Argentina highlands
Autor/es:
MORENO, MARÍA ALEJANDRA; ZAMPINI, IRIS CATIANA; ISLA, MARÍA INÉS
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 2020
ISSN:
0378-8741
Resumen:
Ethnopharmacological relevance: The Argentinean medicinal plants Larrea divaricataCav., Larrea cuneifolia Cav., Larrea nitida Cav., Zuccagnia punctata Cav. andTetraglochin andina Ciald are used alone and in combined in traditional medicine byinhabitants from Argentinean northwestern to solve mycosis, vaginal infections,gastrointestinal, respiratory, and inflammatory process.Aim of the study: To assess the effect of interactions between dry extracts of these fivespecies of medicinal plants against yeast strains isolated from vaginal infections, selectthe most active mixtures and evaluate the anti-inflammatory and antioxidant activities.Material and methods: Synergy between the plants extracts was studied using a brothmicrodilution assay by the checkerboard method against Saccharomyces cerevisiae,Candida albicans, and non albicans strains. The inhibitory effect on lipoxygenaseenzyme and the antioxidant capacity in cell free and cell systems were studied. Thechemical profile was evaluated by qualitative and quantitative screening, whereaschemical markers were quantified by HPLC-DAD.Results: A synergistic antifungal effect was observed in some of the binarycombinations. Z. punctata/ L. divaricata, Z. punctata/ L. cuneifolia, and Z. punctata/ L.nitida were the most active mixtures. Nordihydroguaiaretic acid and 2′,4′-dihydroxychalcone, two antifungal compounds present in these extracts, were identified and quantified by HPLC-DAD. Both the single extracts and the bi-herbal mixtures showed antioxidant activity (in cellular and in cell-free systems) and were active on proinflammatory enzymes (LOX).Conclusions: Our results indicated that the most active combinations of these speciesextracts could be useful in the treatment of vaginal infectious diseases caused bySaccharomyces cerevisiae and Candida spp. strains and in the associated oxidative andinflammatory processes. In addition, the results highlighted the potential phytotherapeuticof total phytochemical compounds present in these medicinal plants