Inhibition of biofilms producer strains of interest industrial.

DÁVILA MARÍA DE LOURDES; ANDUNI GUILLERMO; GUSILS CARLOS; RUIZ MARCELO; CÁRDENAS GERÓNIMO

BIOCELL

Instituto de Histología y Embriología "Dr. Mario H. Burgos". CONICET

Lugar: Mendoza; Año: 2008 vol. 32 p. 93 - 93

0327-9545

The presence of biofilms in food industries implies hygienic problems and economic losses, since products thus polluted are necessarily rejected. The objective was to evaluate the in vitro antimicrobial effect, Minimal Inhibitory Concentration (MIC) and Bactericide Mimimal Concentration (MBC) of diverse microbicidal agents. Biofilm-producing strains isolated from regional factories were studied. To determine MIC values, two microbiological techniques were used: 1) E-test with different concentrations of sodium hypochlorite, malic acid, lactic acid and dimetilditiocarbamato-etilenbisditiocarbamato (DE); 2) serial dilutions (1:2) of substances. To determine MBC values, the negative tubes MIC determination were inoculated in Mueller Hinton agar. The same CIM values for antimicrobial substances were obtained using both microbiological techniques. Of the four antimicrobial agents tested, DE showed higher MIC values for all strains tested. When MBC was determined, it was observed that using lactic acid, malic acid and sodium hypochlorite, values obtained were consistent or varied in a dilution with CIM obtained in the four strains tested. However, in the case of DE, higher concentrations were required to observe a bactericidal effect on the bacterial strains.