INVESTIGADORES
GUEVARA Maria Gabriela
congresos y reuniones científicas
Título:
EVALUATION OF HAEMOSTATIC ACTIVITY OF A SERINE PROTEASE
Autor/es:
PEPE, A.; FERNANDEZ, MB; DALEO GR; GUEVARA, MG
Lugar:
Rosario
Reunión:
Congreso; Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Blood coagulation and fibrinolysis, two important processes associated with
haemostasis and wound healing, involve a series of serine proteases.
Fibrinogenolytic activity and anticoagulant ability of several plant serine
proteases have been reported. Previously, we have reported the purification and
partial characterization of a serine protease from Solanum tuberosum leaves,
named as StSBTc-3. The aim of this study was to evaluate the fibrinogenolytic
and anticoagulant capacity of StSBTc-3. The fibrinogenolytic activity of StSBTc-3
was analyzed by SDS-PAGE 10%, incubating different amounts of StSBTc-3 at
several times (0-6 hours) with human fibrinogen isolated from human serum
plasma. Thrombin time of pooled normal human plasma was determined to
evaluate anticoagulant activity of StSBTc-3. The results obtained here show that
StSBTc-3 is able to degrade human fibrinogen in a dose dependent manner.
Contrary to the results obtained for other plant serine proteases, StSBTc-3 is able
to cleave all chains of human fibrinogen. Thrombin time results show that StSBTc-
3 has anticoagulant activity in a dose dependent manner, being the maximum
effect at 14 ìg/ml like others plant serine proteases reported. The results indicate
that StSBTc-3 may have potential and therapeutic applications.