Arabidopsis thaliana ASPARTIC PROTEASES AND THEIR ROLE IN DROUGHT STRESS

D´IPPÓLITO SEBASTIÁN; FREY, MARÍA EUGENIA; MARÍA G. GUEVARA.

Capital Federal

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Plant aspartic proteases have been implicated in protein processing as well as in plant senescence, stress responses, programmed cell death and reproduction. In last years, it has been reported that they might have a role in the adaptation of plants to an environment with less water availability. In previous results we determined that an A. thaliana gene that encodes for an aspartic protease (At1g11910) that is expressed in guard cell, is induced under deficit water conditions (Fig 1). The aim of this work was to study the role of At1g11910 during the plant response to drought.We evaluated the response of Col-0 and At1g11910 mutant plants in a mild water deficit (MWD) condition. Seedlings of 14 days were putted in pots with an equal substrate quantity and were watered until saturation during 10 days. Then, treated plants were watered until reached a 26% of the maximum substrate capacity (0.13 g of water/g substrate) during the next 20 days. We evaluated the phenotype of each plant under stress conditions and quantified different hydric parameters as water content, consumption and loss.