INVESTIGADORES
GUEVARA Maria Gabriela
congresos y reuniones científicas
Título:
Tuber apoplastic hydrophobic proteins differentially expressed in
Autor/es:
M. B. FERNANDEZ (1), M. G. GUEVARA (1), G. R. DALEO (1), M. R. PAGANO
Lugar:
Oregon, EEUU
Reunión:
Congreso; Annual Meeting of Phytopathological Society (APS); 2009
Resumen:
During plant pathogen interaction, oomycetes secrete effectors into the plant
apoplast where they interact with host resistance proteins, which are
accumulated after wounding or infection. Expression profile of pathogenesis
related proteins is proportional to the resistance of different cultivars towards
P. infestans infection. The aim of this work was to analyse the pattern of
expression of apoplastic hydrophobic proteins (AHPs), after wounding or
infection, in tubers from two potato cultivars with different resistance to P.
infestans. Intercellular fluid was extracted from tubers (IFT) and
chromatographed into a PepRPC HR5-5 column in FPLC eluted with a
linear gradient of 75% acetonitrile. Then, AHPs were analyzed by SDS-PAGE
and identified by MALDI TOF. Results obtained shown that in the resistant
cultivar (Innovator), the AHPs concentration was higher than in the
susceptible cultivar (Spunta), in all times and conditions assayed. Quantitative
and qualitative changes were observed in hydrophobic proteins in both
cultivars after infection. In Spunta cultivar patatin precursors and a protein of
9 KDa were induced after infection. In Innovator cultivar, the levels of
protease inhibitors, patatin precursors and a protein of 16 KDa were increased.
The results obtained here suggest that changes in the AHPs levels could be
necessary to protect the plant against pathogens. Furthermore, those basal
levels of AHPs would be related with the degree resistant to pathogens.