Antiacarsia gemmatalis Nuclear Polyhedrosis virus Replication in Serum Free and Serum Reduced Insect Cell Cultures.

CLAUS JD,; REMONDETTO GE,; GUERRERO S.A; DEMONTE ANA,; MURGUIA M,; MARCIPAR AJ,

JOURNAL OF BIOTECHNOLOGY

Elsevier

Lugar: Holanda; Año: 1993 vol. 31 p. 1 - 15

0168-1656

Anticarsia gemmatalis nuclear polyhedrosis virus replication in serum-free and serum-reduced insect cell cultures Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Juan D. Claus, Gabriel E. Remondetto, Sergio A. Guerrero, Ana M. Demonte, Marcelo Murguía and Alberto J. Marcipar INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina Received 20 July 1992;  accepted 29 September 1992.  Available online 10 December 2002. Abstract In order to develop a financially feasible process to produce Anticarsia gemmatalis Nuclear Polyhedrosis virus in cell culture, we developed a lipidic supplement to replace fetal calf serum in insect cell culture media. The supplement, prepared with an extract of lipids from hen egg yolk, allowed us to reduce the contents of serum in the culture medium from 10% to 1%. IPLB-Sf-21 cells could be kept along consecutive passages in serum-reduced medium. The replication of AgNPV in HEYLE-supplemented cultures was evaluated. Extracellular virions production was the same as in FCS-supplemented-cultures, but the production level of polyhedral inclusion bodies was significantly lowered in HEYLE-supplemented cultures. The reduced production of PIBs is related to a premature releasing of non-occluded particles as well as to a reduced synthesis of polyhedrin protein. Author Keywords: Insect cell culture; Spodoptera frugiperda cells; Serum-free culture; Hen egg yolk lipid; Anticarsia gemmatalis Nuclear Polyhedrosis virus; Autographa californica Nuclear Polyhedrosis virus; Occluded virus; Non-occluded virus; Polyhedrin protein