INVESTIGADORES
GORLA Nora Bibiana Maria
artículos
Título:
Uso del Fingerprinting de ADN para asignar paternidad
Autor/es:
GORLA N, BENAVIDES F, SALAVERRI M,LUDUEÑA R, GRANDOLI N, CORLEY E, LARRIPA I
Revista:
ARCHIVOS DE MEDICINA VETERINARIA
Editorial:
UNIV AUSTRAL CHILE
Referencias:
Año: 1998 vol. 30 p. 151 - 154
ISSN:
0301-732X
Resumen:
The efficiency of DNA fingerprinting to solve a paternity dispute was evaluated in four
Hereford bulls of a Cebú/Hereford cattle herd with two calves affected by a congenital
abdominal wall malformation. The test was carried out using multi-locus probe (CAC)5. 8
DNA samples ?two from the affected calves, two from their corresponding mothers and 4
from bulls (supposed sires)? were processed. These samples were digested with restriction
enzyme Hae III, blotted onto nylon membranes and hybridised with (CAC)5. The bands
obtained were visually analysed and data was processed by the computer program
"PATER" (with the "PATER" computer program). The probability of paternity (W) was W
= 0.80 in one case and W = 0.93 in the other. Despite the known inbreeding of the bovine
breed tested, it was possible to ascertain the paternity with multilocus DNA fingerprinting
(CAC)5.
Palabras claves: Fingerprinting, minisatélites, paternidad, bovinos, malformación
congénita.
Key words: Fingerprinting, minisatellites, paternity, calves, congenital malformation.