INVESTIGADORES
GORLA Nora Bibiana Maria
artículos
Título:
HPLC RESIDUES OF ENROFLOXACIN AND CIPROFLOXACIN IN EGGS OF LAYING HENS
Autor/es:
GORLA N, CHIOSTRI E, UGNIA L, WEYERS A, GIACOMELLI N, DAVICINO R, GARCÍA OVANDO H
Revista:
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 1997 vol. 8 p. 253 - 256
ISSN:
0924-8579
Resumen:
Abstract A technique for the extraction and liquid chromatographic determination of enrofloxacin residues in chicken muscle is described. The muscle samples were extracted with three steps of dichloromethane and centrifugation for the isolation of enrofloxacin from muscle. Enrofloxacin was quantified by liquid chromatography with fluorescence detection at 297 nm excitation and 440 nm emission. The assay is linear from 0.00048 to 0.25 ìg/mL. The limit of detection is 0.00048 ìg/mL and the limit of quantification is 0.0039 ìg/mL. The recoveries of enrofloxacin residues (0.0039 to 0.25 ìg/mL) averaged 86.67%. The injected extracts are free from matrix interferences, making it easy to calculate the amount of residue present. This is a sensitive, economic, and fast method to investigate the presence of enrofloxacin in chicken muscle.
