INVESTIGADORES
GORLA Nora Bibiana Maria
artículos
Título:
INCREASE OF TISSUE LIPID HYDROPEROXIDES AS DETERMINATION OF
Autor/es:
WEYERS A, GORLA N1, UGNIA L, GARCÍA OVANDO H, CHESTA C
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: INCREASE OF TISSUE LIPID HYDROPEROXIDES AS DETERMINATION OF; Año: 2001 p. 11 - 15
ISSN:
0327-9545
Resumen:
Increased levels of lipid hydroperoxides (LOOH) are frequently associated with the oxidative
mechanisms involved in physiological states as ageing and with serious pathological
conditions. In the present work the physiological and the CCl4-induced lipid hydroperoxide
levels in mice liver and kidney were determined. The analysis of LOOH tissue levels was
performed through the oxidation of 1- napthyldiphenilphosphine (NDPP) into its oxide
(ONDPP) and further quantification by high pressure liquid chromatography at 292 nm UV
detection.
The physiological level of lipid hydroperoxides levels was higher in the kidney (245 + 8 nmol
LOOH/ g of tissue) than in liver (164 + 5 nmol of LOOH/ g of tissue). After a single
administration of CCl4 (0,25 ml/ g) tissue LOOH reached a maximun level after 15 min (416 +
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
levels in mice liver and kidney were determined. The analysis of LOOH tissue levels was
performed through the oxidation of 1- napthyldiphenilphosphine (NDPP) into its oxide
(ONDPP) and further quantification by high pressure liquid chromatography at 292 nm UV
detection.
The physiological level of lipid hydroperoxides levels was higher in the kidney (245 + 8 nmol
LOOH/ g of tissue) than in liver (164 + 5 nmol of LOOH/ g of tissue). After a single
administration of CCl4 (0,25 ml/ g) tissue LOOH reached a maximun level after 15 min (416 +
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
4-induced lipid hydroperoxide
levels in mice liver and kidney were determined. The analysis of LOOH tissue levels was
performed through the oxidation of 1- napthyldiphenilphosphine (NDPP) into its oxide
(ONDPP) and further quantification by high pressure liquid chromatography at 292 nm UV
detection.
The physiological level of lipid hydroperoxides levels was higher in the kidney (245 + 8 nmol
LOOH/ g of tissue) than in liver (164 + 5 nmol of LOOH/ g of tissue). After a single
administration of CCl4 (0,25 ml/ g) tissue LOOH reached a maximun level after 15 min (416 +
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.
4 (0,25 ml/ g) tissue LOOH reached a maximun level after 15 min (416 +
21 nmol/g kidney and 303 + 6 nmol/ g liver) and then slowly decreased. LOOH levels in liver
afforded an early indicator (15 min) of oxidative damage. LOOH levels in kidney remained
significatively increased up to 60 min post administration.
The described HPLC assay is a useful, simple and sensitive method to detect cellular oxidative
stress and damage.