AZT-induction of micronuclei in human lymphocyte subpopulations

STERN M, GONZÁLEZ CID M, LARRIPA I, SLAVUTSKY I.

TOXICOLOGY LETTERS

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 1994 vol. 70 p. 235 - 242

0378-4274

Micronucleus (MN) induction by azidothymidine (AZT) in B and T lymphocytes was analyzed by a recently developed MAC (morphology-antibody-chromosome) method which allows the immunologic identification of different cell lineages. An increased frequency of MN in AZT-treated cultures compared with controls was observed. CD4 cells were found to be more sensitive to AZT damage. AZT-treated cultures showed a significant decrease in the proportion of CD4 interphasic cells. Furthermore, higher MN frequencies in isolated lymphocytes than in whole blood in both control and AZT-treated cultures were observed. Se adjunta el trabajo en papel