INVESTIGADORES
GOLDMAN Alejandra
congresos y reuniones científicas
Título:
PLANT HEAT SHOCK PROTEIN 90 AS CARRIER - ADJUVANT FOR IMMUNIZATION AGAINST A REPORTER ANTIGEN
Autor/es:
CORIGLIANO, MARIANA G ; FENOY , IGNACIO ; SANDER, VALERIA ; MAGLIOCO, ANDREA ; GOLDMAN, ALEJANDRA ; CLEMENTE, MARINA
Lugar:
Los Cocos, Córdoba
Reunión:
Congreso; Reunión Anual Sociedad Argentina de Inmunología; 2013
Institución organizadora:
SAI
Resumen:
The main goal of adjuvant research is to find new adjuvants able to
stimulate Th1, Th2 or both responses
but with reduced toxicity.In this context, the study of the
immunomodulatory properties of molecules and
proteins derived from different organisms can contribute to find new and
more efficient adjuvants.
Heat Shock Proteins (HSPs) such as Hsp60, Hsp70 and Hsp90 have been
recently reported to play important roles in antigen presentation, activation
of lymphocytes and macrophages, and maturation of dendritic cells. Therefore,
plant Hsp90s are an attractive option to use as novel and interesting carriers-
adjuvants for proteins or peptides of immunoprotective value. In the
present study, we evaluated the modulation of the immune response induced by Hsp90
of Nicotiana benthamiana(NbHsp90.3) against the Maltose Binding Protein (MBP)
as a reporter antigen. Equimolar quantities of recombinant proteins were
administered in mice as follows: MBP alone (MBP group), a mixture of MBP and
rNbHsp90.3 (MBP+rNbHsp90.3 group) and the fusion of MBP to rNbHsp90.3
(MBP-rNbHsp90.3 group). Although
a significative increase of MHC class I surface molecules was observed in
MBP+rNbHsp90.3 and MBP-
rNbHsp90.3 groups, the covalent linkage between NbHsp90.3 and MBP to bring
a fusion protein was
essential to induce the strong specific antibody response with predominance
of IgG2a. Eighty-four days
after the first immunization, splenocyte proliferation from
MBP-rNbHsp90.3-immunized mice was
consistently higher than those from MBP and MBP+rNbHsp90.3 groups. In
addition, splenocytes from
MBP-rNbHsp90.3 immunized mice produced higher levels of IFN-gamma than
controls. Finally, the
percentage of CD8+T cells significantly
increased in MBP-rNbHsp90.3-immunized mice. Taken together,
these results suggest that plant HSP90s could be incorporated as adjuvants
in vaccines that require the
generation of a Th1 response along with a CD8 cytotoxic cell response to
confer immunity.