Antihistamines and corticoids co-treatment rationale. Potentiation of glucocorticoids antiinflammatory effects

ZAPPIA, CD; SOTO, A; GRANJA GALEANO; FERNANDEZ, N; SHAYO, C; GOLDMAN A; FITZSIMONS CP; MONCZOR, F

Congreso; Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016

Antihistamines and corticoids are used to treat numerous inflammatory conditions, such as allergic rhinitis or asthma respectively, and in many cases they are co-administered. However, this association has no clear rationale and has arisen from clinical practice. We have previously reported the potentiating effects of histamine H1 receptor (H1R) signaling on glucocorticoid receptor (GR) activity in vitro. Here we evaluated the effect of the antihistamine azelastine (Aze) on dexamethasone (Dex) induced GR transcriptional activity. HEK293T cells were co-transfected with plasmids coding for GR, H1R and a luciferase reporter plasmid regulated by the GR to study its transactivation (TAT3-Luc) or its transrepression (IL6-Luc). In both cases, preincubation of cells with Aze 10 μM induced a three-fold increment in the response elicited by Dex 0.1 nM (p