INVESTIGADORES
GOLDMAN Alejandra
congresos y reuniones científicas
Título:
Plant hsp90 proteins interact with b-cells and stimulate their proliferation
Autor/es:
CORIGLIANO MG, ALASINO RV, MAGLIOCO A, LAGUIA BECHER M, GOLDMAN A, MARTIN V, ANGEL SO, CLEMENTE M
Lugar:
San Luis
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Resumen:
BT-C01.
PLANT HSP90 PROTEINS INTERACT WITH B-CELLS
AND STIMULATE THEIR PROLIFERATION
Corigliano MG1, Maglioco A2, Laguia Becher M1, Goldman A3,
Martin V3, Angel SO4, Clemente M1.
1Lab Biotecnología Vegetal, IIB-INTECH. 2ILEX. 3CESyMA. 4Lab
Parasitología Molecular, IIB-INTECH. Chascomús, Buenos Aires.
E-mail: mclemente@intech.gov.ar
The molecular chaperone Hsp90 plays an important role in folding
stabilization and activation of client proteins, also Hsp90 of
mammals and mammalian pathogens displays immunostimulatory
properties. We investigated the role of plant-derived Hsp90s as Bcell
mitogens by measuring their proliferative responses in vitro.
Plant cytosolic Hsp90 isoforms from Arabidopsis thaliana
(AtHsp81.2) and Nicotiana benthamiana (NbHsp90.3) were
expressed in E.coli. rAtHsp81.2 and rNbHsp90.3 proteins induced
prominent proliferative responses in spleen cells form BALB/c
mice. In vitro incubation of spleen cells with rpHsp90 led to the
expansion of B lymphocytes. This effect was confirmed by
immunofluorescence analysis, where a direct binding of rpHsp90 to
B- but not to T-cells was observed in cells from BALB/c and
C3H/HeN mice. Finally, we examined the involvement of Toll Like
Receptor 4 (TLR4) in the rpHsp90s induction of B-cell proliferation.
Spleen cells from C3H/HeJ mice responded poorly to prAtHsp90.
However, the interaction between rpHsp90 and B-cells from
C3H/HeJ mice was not altered, suggesting that the mutation on
TLR4 would be affecting the signal cascade but not the rpHsp90-
TLR4 receptor interaction. Our results show that spleen cell
proliferation can be stimulated by a non-pathogen-derived Hsp90.
Furthermore, our data provide a new example of a non-pathogenderived
ligand for TLRs