Effect of respirable dust emitted from unpaved rural roads on skin and innate immune response.

C AZZI; I FENOY; T ULCHAK; N ORONA; V MARTÍN; RAMIREZ HABERKON NANCY; M MENDEZ; J PANEBIANCO; A GOLDMAN; F ASTORT

Mar del Plata

Congreso; Reunión Anual Biociencias-SAI-FAIC, 2022; 2022

Environmental factors such as air particulate matter (PM) are involvedin the development of allergic disorders like atopic dermatitis.The increase of allergic diseases observed in rural areas may be aconsequence of respiratory and dermal exposure to air PM containinginorganic and organic soil elements as well as agrochemicals.The main source of rural PM is wind erosion of soils used for intensivecultivation. Also unpaved rural roads are an important sourceof PM emission. Although several studies related the exposure tourban PM with the onset or exacerbation of allergies such as atopicdermatitis, there are few studies that link rural PM with the developmentof disorders. Therefore, we aim to study the effect of PM emittedfrom unpaved roads from intensive cultivation areas where theuse of agrochemicals prevails, on innate immune response. AdultBALB/c mice were exposed to PM emitted from unpaved roads frominside (PM in) or outside (PM out) of a farm field by simultaneous intranasalinstillation (1mg/kg BW) and dermal exposure (16 μg/cm2).After 24h, ILC2 population was assessed by flow cytometry and cytokine(IL-10, IL-5, IFN-Ɣ and IL-6) levels were analyzed in supernatantsfrom splenocytes and brachial draining lymph node cells (DLN)stimulated ex vivo with PMA/Ionomycin by ELISA. Mice exposed toPM in showed increased IL-6 (p