Biological effect of respirable dust emitted fr.om unpaved rural roads of Argentina on lung and skin cells

TATIANA ULCHAK; F ASTORT; I FENOY; I PALAVECINO; RAMIREZ HABERKON NANCY ; M MENDEZ; A RANDI; J PANEBIANCO; A GOLDMAN; N ORONA

Mar del Plata

Congreso; Reunión Anual Biociencias-SAI-FAIC, 2022; 2022

Air particulate matter (PM) is an environmental health problem affectingthe world’s population. Although several studies related theexposure to urban PM with the onset or exacerbation of allergiessuch as asthma and atopic dermatitis, there are few studies thatlink rural PM with the development of these allergic disorders. RuralPM comes mainly from agricultural activity, where agrochemicalcompounds are frequently used. Also it is known that unpaved roadsexposed to wind erosion are an important source of air PM. Therefore,the aim of this study was to evaluate the effect of respirabledust emitted from unpaved roads from intensive cultivation areaswhere the use of agrochemicals prevails from the central Pampasregion on pulmonary (A549) and skin (HaCaT) cells. A549 or HaCaTcells were exposed to PM (1-100μg/ml) emitted from unpaved roadsfrom inside (high glyphosate/AMPA) or outside (low Gliphosate/AMPA) of a farm field. After 24h, metabolic activity (MTT), lactatedehydrogenase activity (LDH) and IL-8 cytokine release (ELISA)was assessed. We found that A549 cells exposed to PM showed amarked increase in IL-8 production for both particles (P