INVESTIGADORES
GIORDANO Walter Fabian
artículos
Título:
Purification and partial characterization of novel bacteriocin L23 produced by Lactobacillus fermentum L23.
Autor/es:
PASCUAL, L., DANIELE, M., GIORDANO, W., PÁJARO, M. AND BARBERIS, L.
Revista:
CURRENT MICROBIOLOGY
Editorial:
Springer
Referencias:
Año: 2008 vol. 56 p. 397 - 402
ISSN:
0343-8651
Resumen:
Lactobacillus fermentum strain L23 produced a small bacteriocin, designated bacteriocin L23, with an estimated molecular mass of <7,000 Da. Isolation, purification, and partial characterization of bacteriocin L23 are described. It displayed a wide inhibitory spectrum including both Gram-negative and Gram-positive pathogenic strains, and two species of Candida. The antibacterial activity of cell-free culture supernatant fluid was not affected by catalase or urease, but was abolished by the proteolytic enzymes trypsin and protease VI. Bacteriocin L23 was heat-stable (60 min at 100ºC) and showed inhibitory activity over a wide pH range (4.0 to 7.0). The proteinaceous compound was isolated from cell-free culture supernatant fluid and purified. Crude bacteriocin sample was prepared by a process of ammonium sulfate precipitation, gel filtration, thin-layer chromatography (TLC), bioautography, and reversed-phase HPLC.
