INVESTIGADORES
GARRAMUÑO Patricia
congresos y reuniones científicas
Título:
Hsp25 and Hsp70 Expression in Neonatal Obstructive Nephropathy
Autor/es:
RINALDI TOSSI MARTIN; MONTT GUEVARA MOAGDALENA; GARRAMUÑO VALLÉS PATRICIA; VARGAS ROIG LAURA
Lugar:
Mendoza, Argentina
Reunión:
Congreso; XXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo y I Reunión de la Dirección de Investigación, Ciencia y Técnica dependiente del Ministerio de Salud Gobierno de Mendoza; 2008
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
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Chronic and
complete unilateral ureteral obstruction (UUO) in the neonatal rat causes
delayed renal maturation, tubular apoptosis, and interstitial inflammation.
This obstruction induces oxidative stress in both the obstructed (O) and
the contralateral unobstructed (CL) kidney. Experimental
evidences have shown that there exists an interrelationship between Hsp25, Hsp70
and redox status. In the present study we examined the expression of
these Hsps in control (C), O and CL kidneys. Neonatal rats were subjected to
UUO (n=5) within the first 48 h of life. After 7, 10 sn14 days of obstruction,
animals were sacrified and their kidneys were decapsulated and removed. The
expression of Hsps were studied by immunohistochemistry and western blot. In C
(n=4), Hsp25 was observed mainly in the cytoplasm of collecting ducts in inner
medulla. Hsp25 expression was not induced in O kidney. In C kidney, Hsp70 expression was observed in the
cytoplasm of a high percent of tubular epithelial cells in cortex. The
expression of Hsp70 in C kidneys was weakly in most of the tubules in outer
medulla, whereas in the papilla intense staining of collecting ducts was noted.
In O kidney we did not observe increased expression of Hsp70. In CL (n=2) kidneys
a high hsp70 expression was observed in tubular epithelial cells of cortex and
in papilla.
Conclusion:We
demonstrated Hsp70 upregulation in contralateral cortex tubular cells,
suggesting a possible cytoprotective role of this chaperone in unilateral obstructive nephropathy.
