Cytoprotective Effects Of Hsp70 And Transcription Factor Nrf2 IN Neonatal Obstructive Nephropathy

RINALDI TOSI MARTÍN; BOCANEGRA VICTORIA; MANUCHA WALTER; BENARDON MARIA EUGENIA; GARRAMUÑO VALLÉS, PATRICIA

La Punta ,San Luis

Congreso; XXVII Reunión Científica Anual Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

CYTOPROTECTIVE EFFECTS OF Hsp70 AND ANTIOXIDANT TRANSCRIPTION FACTOR Nrf2 IN NEONATAL OBSTRUCTIVE NEPHROPATHY Rinaldi Tosi ME, Bocanegra V, Manucha W, Benard¨®n ME, Vall¨¦s P. IMBECU-CONICET-UNCuyo. E-mail: rinaldi@lab.cricyt.edu.ar An important mechanism by which cells adapt to oxidant stress is to transcriptionally up-regulate a distinct array of cytoprotective genes responsible for buffering the cells¡¯ antioxidant capacity. In this study, we examined Nrf2 transcription factor and Hsp70 expression on oxidative stress modulation in neonatal unilateral ureteral obstruction (UUO). Rats were subjected to UUO (n=5) within the first 48h of life. After 7, 10 and 14 days of obstruction, nephrectomy was performed. The expression of Hsp70, Nrf2 and Keap1 were studied by WB; NADPH-oxidase and total antioxidant status (TAS) activity were performed. After 14 days of obstruction, decreased Hsp70 protein expression associated with Nrf2 protein cytosolic downregulation, linked to increased Keap1 expression, were shown. Oxidative stress induction was shown by NADPH oxidase activity and decreased TAS 14 days after obstruction. Conversely, 7 and 10 days after kidney obstruction, increased Hsp70 expression and cytosolic Keap1 linked to nuclear Nrf2 expression that leads to enhanced antioxidant glutathione S-transferase (GSTA2) gene with absence of oxidative response was demonstrated. These findings suggest that induction of Hsp70 and Nrf2 expression are involved on obstruction- oxidative stress resistance. (GSTA2) gene with absence of oxidative response was demonstrated. These findings suggest that induction of Hsp70 and Nrf2 expression are involved on obstruction- oxidative stress resistance. (GSTA2) gene with absence of oxidative response was demonstrated. These findings suggest that induction of Hsp70 and Nrf2 expression are involved on obstruction- oxidative stress resistance. S-transferase (GSTA2) gene with absence of oxidative response was demonstrated. These findings suggest that induction of Hsp70 and Nrf2 expression are involved on obstruction- oxidative stress resistance. 138. RED WINE POLYPHENOLS SYNERGISTICALLY INHIBIT ANGIOTENSIN II-INDUCED CELL MIGRATION AND REACTIVE OXYGEN SPECIES PRODUCTION Redondo A, Estrella N, Castro C, Cruzado M. Program I+D SeCTyP, FCM, UNCuyo, IMBECU, CONICET. Email: mcruzado@fcm.uncu.edu.ar Dietary polyphenols are associated with the prevention of arterial hypertension. Angiotensin II (AII) is an important humoral factor associated with this disease. Reactive oxygen species (ROS) are involved in AII-induced vascular effects. We studied the effect of antioxidants present in red wine, quercetin (Q) and catechin (C), on AII-induced cell migration and ROS production in vascular smooth muscle cells (VSMC) obtained from small arteries of spontaneously hypertensive rats (SHR). We determined cell migration by scrape-wound assay, and ROS production by incubation with a fluorescent probe (H2DCF-DA). Data (mean¡ÀSEM) were analyzed by ANOVA and post test of Bonferroni. Q (10, 20 and 30 ¦Ìg/mL) inhibited AII (10-7M) induced VSMC migration after 24 h stimulation but C had no effect at the used concentrations. However, coincubation of Q(5¦Ìg/mL) plus C (6¦Ìg/mL) significantly inhibited AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. but C had no effect at the used concentrations. However, coincubation of Q(5¦Ìg/mL) plus C (6¦Ìg/mL) significantly inhibited AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. but C had no effect at the used concentrations. However, coincubation of Q(5¦Ìg/mL) plus C (6¦Ìg/mL) significantly inhibited AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. inhibited AII (10-7M) induced VSMC migration after 24 h stimulation but C had no effect at the used concentrations. However, coincubation of Q(5¦Ìg/mL) plus C (6¦Ìg/mL) significantly inhibited AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. AII-induced migration (157%¡À15.8 vs. 31.7%¡À7.2; p<0,001), suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. suggesting a cooperative effect of polyphenols. Similarly, AII-stimulated ROS production was significantly inhibited by the combination of Q plus C (2.43 URF¡À0.33 vs. 0.60 URF¡À0.30; P<0.001). Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. Our data suggest that polyphenols present in red wine may reduce in a synergistic way AII-induced oxidative stress and VSMC migration, preventing vascular damage in hypertension. but C had no effect at the used concentrations. However,